ID P33MX_HUMAN Reviewed; 305 AA. AC Q96A73; B2RD69; B8K1S6; Q6IA24; Q8NDU3; Q9BRE5; Q9H7D5; Q9ULM9; DT 23-OCT-2007, integrated into UniProtKB/Swiss-Prot. DT 01-DEC-2001, sequence version 1. DT 28-JAN-2026, entry version 161. DE RecName: Full=Putative monooxygenase p33MONOX; DE EC=1.-.-.-; DE AltName: Full=Brain-derived rescue factor p60MONOX; DE AltName: Full=Flavin monooxygenase motif-containing protein of 33 kDa; GN Name=KIAA1191; Synonyms=P33MONOX; OS Homo sapiens (Human). OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; OC Homo. OX NCBI_TaxID=9606; RN [1] RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). RA Heese K.; RT "Brain-derived rescue factor p60MONOX."; RL Submitted (MAY-2005) to the EMBL/GenBank/DDBJ databases. RN [2] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). RC TISSUE=Brain; RX PubMed=14702039; DOI=10.1038/ng1285; RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S., RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., RA Isogai T., Sugano S.; RT "Complete sequencing and characterization of 21,243 full-length human RT cDNAs."; RL Nat. Genet. 36:40-45(2004). RN [3] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). RA Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.; RT "Cloning of human full open reading frames in Gateway(TM) system entry RT vector (pDONR201)."; RL Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases. RN [4] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). RC TISSUE=Brain, and Small intestine; RX PubMed=17974005; DOI=10.1186/1471-2164-8-399; RA Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., RA Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., RA Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., RA Wiemann S., Schupp I.; RT "The full-ORF clone resource of the German cDNA consortium."; RL BMC Genomics 8:399-399(2007). RN [5] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RX PubMed=15372022; DOI=10.1038/nature02919; RA Schmutz J., Martin J., Terry A., Couronne O., Grimwood J., Lowry S., RA Gordon L.A., Scott D., Xie G., Huang W., Hellsten U., Tran-Gyamfi M., RA She X., Prabhakar S., Aerts A., Altherr M., Bajorek E., Black S., RA Branscomb E., Caoile C., Challacombe J.F., Chan Y.M., Denys M., RA Detter J.C., Escobar J., Flowers D., Fotopulos D., Glavina T., Gomez M., RA Gonzales E., Goodstein D., Grigoriev I., Groza M., Hammon N., Hawkins T., RA Haydu L., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., RA Lopez F., Lou Y., Martinez D., Medina C., Morgan J., Nandkeshwar R., RA Noonan J.P., Pitluck S., Pollard M., Predki P., Priest J., Ramirez L., RA Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., Thayer N., RA Tice H., Tsai M., Ustaszewska A., Vo N., Wheeler J., Wu K., Yang J., RA Dickson M., Cheng J.-F., Eichler E.E., Olsen A., Pennacchio L.A., RA Rokhsar D.S., Richardson P., Lucas S.M., Myers R.M., Rubin E.M.; RT "The DNA sequence and comparative analysis of human chromosome 5."; RL Nature 431:268-274(2004). RN [6] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., RA Hunkapiller M.W., Myers E.W., Venter J.C.; RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases. RN [7] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3). RC TISSUE=Brain, Muscle, and Ovary; RX PubMed=15489334; DOI=10.1101/gr.2596504; RG The MGC Project Team; RT "The status, quality, and expansion of the NIH full-length cDNA project: RT the Mammalian Gene Collection (MGC)."; RL Genome Res. 14:2121-2127(2004). RN [8] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 206-305 (ISOFORM 1). RC TISSUE=Brain; RX PubMed=10574461; DOI=10.1093/dnares/6.5.329; RA Hirosawa M., Nagase T., Ishikawa K., Kikuno R., Nomura N., Ohara O.; RT "Characterization of cDNA clones selected by the GeneMark analysis from RT size-fractionated cDNA libraries from human brain."; RL DNA Res. 6:329-336(1999). RN [9] RP TISSUE SPECIFICITY. RX PubMed=17032350; DOI=10.1111/j.1365-2362.2006.01722.x; RA Yokota T., Mishra M., Akatsu H., Tani Y., Miyauchi T., Yamamoto T., RA Kosaka K., Nagai Y., Sawada T., Heese K.; RT "Brain site-specific gene expression analysis in Alzheimer's disease RT patients."; RL Eur. J. Clin. Invest. 36:820-830(2006). RN [10] RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. RX PubMed=19413330; DOI=10.1021/ac9004309; RA Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.; RT "Lys-N and trypsin cover complementary parts of the phosphoproteome in a RT refined SCX-based approach."; RL Anal. Chem. 81:4493-4501(2009). RN [11] RP INTERACTION WITH NELFB; NOL12 AND PRNP. RX PubMed=21153684; DOI=10.1007/s11010-010-0690-4; RA Mishra M., Inoue N., Heese K.; RT "Characterizing the novel protein p33MONOX."; RL Mol. Cell. Biochem. 350:127-134(2011). RN [12] RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-182 AND SER-183, AND RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. RC TISSUE=Cervix carcinoma, and Erythroleukemia; RX PubMed=23186163; DOI=10.1021/pr300630k; RA Zhou H., Di Palma S., Preisinger C., Peng M., Polat A.N., Heck A.J., RA Mohammed S.; RT "Toward a comprehensive characterization of a human cancer cell RT phosphoproteome."; RL J. Proteome Res. 12:260-271(2013). RN [13] RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-183, AND IDENTIFICATION BY RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. RC TISSUE=Liver; RX PubMed=24275569; DOI=10.1016/j.jprot.2013.11.014; RA Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L., RA Ye M., Zou H.; RT "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver RT phosphoproteome."; RL J. Proteomics 96:253-262(2014). CC -!- FUNCTION: Potential NADPH-dependent oxidoreductase. May be involved in CC the regulation of neuronal survival, differentiation and axonal CC outgrowth. CC -!- SUBUNIT: Interacts with NELFB, NOL12 and PRNP. CC {ECO:0000269|PubMed:21153684}. CC -!- INTERACTION: CC Q96A73; Q9NUX5: POT1; NbExp=2; IntAct=EBI-725897, EBI-752420; CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}. CC -!- ALTERNATIVE PRODUCTS: CC Event=Alternative splicing; Named isoforms=3; CC Name=1; CC IsoId=Q96A73-1; Sequence=Displayed; CC Name=2; CC IsoId=Q96A73-2; Sequence=VSP_028801; CC Name=3; CC IsoId=Q96A73-3; Sequence=VSP_028800; CC -!- TISSUE SPECIFICITY: Down-regulated in the occipital lobe of an early CC stage Alzheimer disease patients. {ECO:0000269|PubMed:17032350}. CC -!- SIMILARITY: Belongs to the P33MONOX family. {ECO:0000305}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; DQ073392; AAY82896.1; -; mRNA. DR EMBL; AK024675; BAB14957.1; -; mRNA. DR EMBL; AK315428; BAG37816.1; -; mRNA. DR EMBL; CR457331; CAG33612.1; -; mRNA. DR EMBL; AL390217; CAB99231.1; -; mRNA. DR EMBL; BX648859; CAH56134.1; -; mRNA. DR EMBL; AC138956; -; NOT_ANNOTATED_CDS; Genomic_DNA. DR EMBL; CH471195; EAW85087.1; -; Genomic_DNA. DR EMBL; BC006316; AAH06316.1; -; mRNA. DR EMBL; BC010448; AAH10448.1; -; mRNA. DR EMBL; BC012848; AAH12848.1; -; mRNA. DR EMBL; BC017097; AAH17097.1; -; mRNA. DR EMBL; AB033017; BAA86505.1; -; mRNA. DR CCDS; CCDS43402.1; -. [Q96A73-2] DR CCDS; CCDS4399.1; -. [Q96A73-1] DR RefSeq; NP_001073152.1; NM_001079684.3. [Q96A73-2] DR RefSeq; NP_001073153.1; NM_001079685.3. [Q96A73-1] DR RefSeq; NP_001274264.1; NM_001287335.1. DR RefSeq; NP_001274265.1; NM_001287336.2. [Q96A73-2] DR RefSeq; NP_065177.2; NM_020444.4. [Q96A73-1] DR RefSeq; XP_005266003.1; XM_005265946.5. [Q96A73-2] DR RefSeq; XP_054208921.1; XM_054352946.1. [Q96A73-2] DR AlphaFoldDB; Q96A73; -. DR SMR; Q96A73; -. DR BioGRID; 121428; 51. DR FunCoup; Q96A73; 194. DR IntAct; Q96A73; 95. DR MINT; Q96A73; -. DR STRING; 9606.ENSP00000298569; -. DR GlyGen; Q96A73; 4 sites, 1 O-linked glycan (3 sites). DR iPTMnet; Q96A73; -. DR PhosphoSitePlus; Q96A73; -. DR BioMuta; KIAA1191; -. DR DMDM; 74731096; -. DR jPOST; Q96A73; -. DR MassIVE; Q96A73; -. DR PaxDb; 9606-ENSP00000298569; -. DR PeptideAtlas; Q96A73; -. DR ProteomicsDB; 75930; -. [Q96A73-1] DR ProteomicsDB; 75931; -. [Q96A73-2] DR ProteomicsDB; 75932; -. [Q96A73-3] DR Pumba; Q96A73; -. DR Antibodypedia; 45989; 89 antibodies from 20 providers. DR DNASU; 57179; -. DR Ensembl; ENST00000298569.9; ENSP00000298569.4; ENSG00000122203.16. [Q96A73-1] DR Ensembl; ENST00000393725.6; ENSP00000377326.2; ENSG00000122203.16. [Q96A73-2] DR Ensembl; ENST00000510164.5; ENSP00000421061.1; ENSG00000122203.16. [Q96A73-1] DR Ensembl; ENST00000614420.4; ENSP00000479627.1; ENSG00000122203.16. [Q96A73-2] DR GeneID; 57179; -. DR KEGG; hsa:57179; -. DR MANE-Select; ENST00000298569.9; ENSP00000298569.4; NM_020444.5; NP_065177.2. DR UCSC; uc003mdw.5; human. [Q96A73-1] DR AGR; HGNC:29209; -. DR ClinPGx; PA143485516; -. DR CTD; 57179; -. DR DisGeNET; 57179; -. DR GeneCards; KIAA1191; -. DR HGNC; HGNC:29209; KIAA1191. DR HPA; ENSG00000122203; Low tissue specificity. DR OpenTargets; ENSG00000122203; -. DR VEuPathDB; HostDB:ENSG00000122203; -. DR eggNOG; ENOG502QRB0; Eukaryota. DR GeneTree; ENSGT00390000000537; -. DR HOGENOM; CLU_079377_0_0_1; -. DR InParanoid; Q96A73; -. DR OMA; TIQAYKG; -. DR OrthoDB; 8935954at2759; -. DR PAN-GO; Q96A73; 1 GO annotation based on evolutionary models. DR PhylomeDB; Q96A73; -. DR PathwayCommons; Q96A73; -. DR SignaLink; Q96A73; -. DR Agora; ENSG00000122203; -. DR BioGRID-ORCS; 57179; 17 hits in 1152 CRISPR screens. DR ChiTaRS; KIAA1191; human. DR GenomeRNAi; 57179; -. DR Pharos; Q96A73; Tdark. DR PRO; PR:Q96A73; -. DR Proteomes; UP000005640; Chromosome 5. DR RNAct; Q96A73; protein. DR Bgee; ENSG00000122203; Expressed in kidney epithelium and 190 other cell types or tissues. DR ExpressionAtlas; Q96A73; baseline and differential. DR GO; GO:0005737; C:cytoplasm; ISS:UniProtKB. DR GO; GO:0005739; C:mitochondrion; HTP:FlyBase. DR GO; GO:0016491; F:oxidoreductase activity; IEA:UniProtKB-KW. DR InterPro; IPR026759; P33MONOX. DR PANTHER; PTHR28342; MONOOXYGENASE P33MONOX-RELATED; 1. DR PANTHER; PTHR28342:SF1; MONOOXYGENASE P33MONOX-RELATED; 1. DR Pfam; PF15302; P33MONOX; 1. PE 1: Evidence at protein level; KW Alternative splicing; Cytoplasm; NADP; Oxidoreductase; Phosphoprotein; KW Proteomics identification; Reference proteome. FT CHAIN 1..305 FT /note="Putative monooxygenase p33MONOX" FT /id="PRO_0000307730" FT REGION 37..56 FT /note="Disordered" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT REGION 158..236 FT /note="Disordered" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT REGION 259..305 FT /note="Disordered" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT MOTIF 67..77 FT /note="Flavin-containing monooxygenase motif" FT COMPBIAS 169..183 FT /note="Low complexity" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT COMPBIAS 193..210 FT /note="Polar residues" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT MOD_RES 44 FT /note="Phosphothreonine" FT /evidence="ECO:0000250|UniProtKB:Q9DBN4" FT MOD_RES 175 FT /note="Phosphothreonine" FT /evidence="ECO:0000250|UniProtKB:Q9DBN4" FT MOD_RES 182 FT /note="Phosphoserine" FT /evidence="ECO:0007744|PubMed:23186163" FT MOD_RES 183 FT /note="Phosphoserine" FT /evidence="ECO:0007744|PubMed:23186163, FT ECO:0007744|PubMed:24275569" FT VAR_SEQ 1..207 FT /note="Missing (in isoform 3)" FT /evidence="ECO:0000303|PubMed:15489334" FT /id="VSP_028800" FT VAR_SEQ 1..19 FT /note="Missing (in isoform 2)" FT /evidence="ECO:0000303|PubMed:17974005" FT /id="VSP_028801" FT CONFLICT 56 FT /note="K -> R (in Ref. 2; BAB14957)" FT /evidence="ECO:0000305" FT CONFLICT 97 FT /note="V -> A (in Ref. 4; CAB99231)" FT /evidence="ECO:0000305" FT CONFLICT 225 FT /note="L -> P (in Ref. 3; CAG33612)" FT /evidence="ECO:0000305" FT CONFLICT 250 FT /note="P -> S (in Ref. 4; CAB99231)" FT /evidence="ECO:0000305" SQ SEQUENCE 305 AA; 33247 MW; 0BC0519F401593CF CRC64; MASRQPEVPA LEASAPLGKM SLPIGIYRRA VSYDDTLEDP APMTPPPSDM GSVPWKPVIP ERKYQHLAKV EEGEASLPSP AMTLSSAIDS VDKVPVVKAK ATHVIMNSLI TKQTQESIQH FERQAGLRDA GYTPHKGLTT EETKYLRVAE ALHKLKLQSG EVTKEERQPA SAQSTPSTTP HSSPKQRPRG WFTSGSSTAL PGPNPSTMDS GSGDKDRNLS DKWSLFGPRS LQKYDSGSFA TQAYRGAQKP SPLELIRAQA NRMAEDPAAL KPPKMDIPVM EGKKQPPRAH NLKPRDLNVL TPTGF //